Saturday, November 23, 2013

DNA Replication

DNA replication is needed for the process mitosis, cell reproduction. DNA replication is a five step process that uses five different enzymes and reads from the 3 carbon end(OH-) to the 5 carbon end. The first enzyme is helicase. The helicase breaks hydrogen bonds between nitrogen bases at the origin of replication, where there are a lot of A-T repeats. The second enzyme is RNA primase, RNA primase creates a polar end for DNA Polymerase 3 (polar), and lays down RNA nucleotides. The next enzyme DNA Polymerase 3 puts in complementary DNA. DNA polymerase 1 replaces the RNA  with DNA. And the final enzyme Ligase glues the Okazaki fragments together.
                                                                                 

 
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